The Immunological Properties of Recombinant Multi-Cystatin-Like Domain Protein From Trichinella Britovi Produced in Yeast.
Trichinellosis is a parasitic disease globally-distributed zoonosis caused by nematodes of the genus Trichinella. One of the most common species are known to affect human health Trichinella is T. britovi; However, it is relatively poorly investigated. A thorough knowledge of the protein expressed by Trichinella important when developing immunological detection methods and vaccines and study the interaction with the host. This study using Pichia pastoris expression Caprine Recombinant Proteins system to produce TbCLP soluble antigen that induces a strong antibody response in the host during a natural infection. Our results demonstrate the feasibility of the production of antigen TbCLP in yeast, which is able to carry out post-translational modifications such as glycosylation and formation of disulfide bonds; they also indicate that the glycosylation TbCLP antigen having immunogenic effects in mice are tested and trigger the Th1 / Th2 mixture, and is associated with reduced ...